1K28
The Structure of the Bacteriophage T4 Cell-Puncturing Device
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9786, 0.9783, 1.0188, 0.9414 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 139.261, 139.261, 381.998 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.21 * |
| Rwork | 0.211 |
| R-free | 0.28000 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.289 |
| Number of reflections | 31712 | |
| <I/σ(I)> | 11.6 | 10.3 |
| Completeness [%] | 99.0 | 99.4 |
| Redundancy | 5.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 12 * | PEG 8000, Tris, Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 5.2 (%) | |
| 2 | 1 | reservoir | Tris | 0.65 (M) | pH8.5 |
| 3 | 1 | reservoir | glycerol | 35 (%) |
