1K28

The Structure of the Bacteriophage T4 Cell-Puncturing Device

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9786, 0.9783, 1.0188, 0.9414
Spacegroup name	H 3 2
Unit cell lengths	139.261, 139.261, 381.998
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.21 *
Rwork	0.211
R-free	0.28000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.081	0.289
Number of reflections	31712
<I/σ(I)>	11.6	10.3
Completeness [%]	99.0	99.4
Redundancy	5.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	12 *	PEG 8000, Tris, Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	5.2 (%)
2	1	reservoir	Tris	0.65 (M)	pH8.5
3	1	reservoir	glycerol	35 (%)