1K1K

Structure of Mutant Human Carbonmonoxyhemoglobin C (beta E6K) at 2.0 Angstrom Resolution in Phosphate Buffer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-03-08
Detector	SIEMENS X1000
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	54.160, 54.160, 195.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.500 * - 2.000
Rwork	0.183
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hho
RMSD bond length	0.007
RMSD bond angle	1.120 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.500 *	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.063
Total number of observations	84880 *
Number of reflections	19382
Completeness [%]	94.0	85.16
Redundancy	4.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	batch method	7.35	310	potassium phosphate 1.75M, pH 7.35, batch method, temperature 310K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	potassium phosphate	1.75 (M)	pH7.35