1K0Z
Crystal Structure of the PvuII endonuclease with Pr3+ and SO4 ions bound in the active site at 2.05A.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.740, 105.630, 46.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.430 - 2.050 |
Rwork | 0.190 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pvu |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.430 | 2.110 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 24251 | |
<I/σ(I)> | 7.2 | 1 |
Completeness [%] | 89.3 | 92.3 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 291 | Ammonium Sulfate, Ammonium Acetate, pH 5.0, VAPOR DIFFUSION, temperature 291K |