1K0R
Crystal Structure of Mycobacterium tuberculosis NusA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.009, 0.939 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 88.987, 88.987, 180.208 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.700 |
R-factor | 0.211 * |
Rwork | 0.248 |
R-free | 0.24900 * |
RMSD bond length | 0.005 |
RMSD bond angle | 1.115 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.103 * | 0.544 |
Number of reflections | 82725 | |
<I/σ(I)> | 10.2 | 2.2 |
Completeness [%] | 91.2 * | 78.2 |
Redundancy | 4.4 * | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 291 * | Gopal, B., (2001) Acta Crystallog., D57, 1187. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.1 (M) | |
3 | 1 | reservoir | MES | 0.1 (M) | pH6.1 |