1K0O
Crystal structure of a soluble form of CLIC1. An intracellular chloride ion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.408, 60.311, 89.428 |
| Unit cell angles | 90.00, 92.41, 90.00 |
Refinement procedure
| Resolution | 87.710 - 1.750 |
| R-factor | 0.243 * |
| Rwork | 0.240 |
| R-free | 0.27200 * |
| Structure solution method | MIR |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 1.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.044 | 0.460 |
| Total number of observations | 212823 * | |
| Number of reflections | 48235 * | |
| <I/σ(I)> | 9.7 | 1.8 |
| Completeness [%] | 98.2 * | 97 * |
| Redundancy | 4.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 4 * | PEG MME 5K, ammonium sulphate, sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-20 (mg/ml) | |
| 2 | 1 | drop | n-octyl-beta-D-glucopyranoside | 0.5 (%) | |
| 3 | 1 | reservoir | PEG5000 MME | 14-18 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0-30 (mM) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
