1K0L
Pseudomonas aeruginosa phbh R220Q free of p-OHB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-06-17 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.875, 138.882, 92.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.600 * - 2.000 |
R-factor | 0.209 * |
Rwork | 0.209 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 22.900 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.600 * | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.096 | 0.789 |
Total number of observations | 261632 * | |
Number of reflections | 30046 | |
Completeness [%] | 96.5 * | 89.7 |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 30 * | 100mM potassium phosphate, 0.05 mM glutathion, 30mM sodium sulfite 0.02mM FAD, 450mM ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | KPi | 100 (mM) | pH7.0 |
3 | 1 | drop | glutathione | 0.05 (mM) | |
4 | 1 | drop | sodium solfite | 30 (mM) | |
5 | 1 | drop | FAD | 0.02 (mM) | |
6 | 1 | drop | ammonium sulfate | 450 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 900 (mM) |