1K0I
Pseudomonas aeruginosa phbh R220Q in complex with 100mM PHB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.678, 138.150, 92.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.000 * - 1.800 |
| R-factor | 0.207 * |
| Rwork | 0.207 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iuw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.300 * |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 * | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.081 | 0.471 |
| Total number of observations | 557623 * | |
| Number of reflections | 38363 | |
| Completeness [%] | 90.6 | 53 |
| Redundancy | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 30 * | 100mM potassium phosphate, 0.05 mM glutathion, 30mM sodium sulfite 0.02mM FAD, 450mM ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | KPi | 100 (mM) | pH7.0 |
| 3 | 1 | drop | glutathione | 0.05 (mM) | |
| 4 | 1 | drop | sodium solfite | 30 (mM) | |
| 5 | 1 | drop | FAD | 0.02 (mM) | |
| 6 | 1 | drop | ammonium sulfate | 450 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 900 (mM) |
