1K06
Crystallographic Binding Study of 100 mM N-benzoyl-N'-beta-D-glucopyranosyl urea to glycogen phosphorylase b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2001-03-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.87 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.653, 128.653, 116.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.840 - 1.800 |
R-factor | 0.215 * |
Rwork | 0.215 |
R-free | 0.24600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1hlf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.840 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.038 * | 0.481 * |
Total number of observations | 668695 * | |
Number of reflections | 88811 | |
<I/σ(I)> | 15.63 | 1.94 |
Completeness [%] | 97.7 | 95 |
Redundancy | 4.9 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 287 | Oikonomakos, N.G., (1985) Biochim. Biophys. Acta, 832, 248. * |