1K05
Crystal structure of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.931 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 87.484, 222.101, 97.885 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.300 - 2.900 |
R-factor | 0.245 * |
Rwork | 0.245 |
R-free | 0.28500 |
Structure solution method | MIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.350 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.300 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.069 * | 0.626 * |
Total number of observations | 48770 * | |
Number of reflections | 20719 | |
<I/σ(I)> | 7.4 | 1.2 |
Completeness [%] | 96.9 | 94.9 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 296 | Hepes, NaCl, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | EDTA | 2 (mM) | |
4 | 1 | drop | dithiothreitol | 2 (mM) | |
5 | 1 | drop | protein | 20 (mg/ml) | |
6 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
7 | 1 | reservoir | 3.3 (M) | ||
8 | 1 | reservoir | glycerol | 1 (%) |