1JZZ
Structural Basis for the Interaction of Antibiotics with the Peptidyl Transferase Center in Eubacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-07-01 |
| Detector | SBC |
| Wavelength(s) | 1.03320 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 170.400, 410.700, 694.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.800 |
| R-factor | 0.209 * |
| Rwork | 0.209 |
| R-free | 0.27400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.660 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.107 * | 0.244 * |
| Number of reflections | 148933 | |
| Completeness [%] | 64.8 | 66.8 |
| Redundancy | 3.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | 291 | ethanol, dimethylhexanediol, MgCl2, KCl, Hepes, NH4Cl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ethanol | ||
| 2 | 1 | 1 | dimethylhexanediol | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 1 | Hepes | ||
| 6 | 1 | 1 | NH4Cl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 10 (mM) | ||
| 2 | 1 | reservoir | 60 (mM) | ||
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | HEPES | 10 (mM) |
