1JZN
crystal structure of a galactose-specific C-type lectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-01-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.8931 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 63.309, 178.486, 137.832 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 2.200 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 24.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.063 * | 0.156 * |
Number of reflections | 38575 | |
Completeness [%] | 96.2 | 79.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | Na acetate pH 4.6, 50 mM lactose, 40 mM CaCl2, 1.5 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-7.5 (mg/ml) | |
2 | 1 | drop | Tris | 10 (mM) | pH8.0 |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | lactose | 100 (mM) | |
5 | 1 | reservoir | 1.3-1.5 (M) | ||
6 | 1 | reservoir | sodium acetate | 1.3-1.5 (M) | pH4.6 |
7 | 1 | reservoir | 40 (mM) |