1JZJ
Pseudomonas aeruginosa Azurin Os(bpy)2(im)(His83)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.873, 54.873, 133.813 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 1.800 |
| R-factor | 0.222 * |
| Rwork | 0.222 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jzi |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.072 * | |
| Number of reflections | 21988 | |
| <I/σ(I)> | 36 | |
| Completeness [%] | 97.4 | 93.9 |
| Redundancy | 8.4 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 298 | PEG, Imidazole, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-30 (mg/ml) | |
| 2 | 1 | drop | imidazole | 40 (mM) | |
| 3 | 1 | drop | 2 (mM) | pH7.2 | |
| 4 | 1 | reservoir | imidazole | 100 (mM) | pH6.0 |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | 6.25 (mM) | ||
| 7 | 1 | reservoir | PEG4000-8000 | 25-38 (%) |
