1JZI
Pseudomonas aeruginosa Azurin Re(phen)(CO)3(His83)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.950, 50.990, 62.440 |
Unit cell angles | 90.00, 128.10, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.600* |
R-factor | 0.227 * |
Rwork | 0.226 |
R-free | 0.24800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jze |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 1.680 |
High resolution limit [Å] | 1.600 * | 1.620 |
Rmerge | 0.080 * | |
Number of reflections | 28956 | |
<I/σ(I)> | 19 | 4.1 |
Completeness [%] | 98.7 | 89.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 293 | PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | drop | imidazole | 40 (mM) | |
3 | 1 | drop | 2 (mM) | pH7.2 | |
4 | 1 | reservoir | imidazole | 100 (mM) | pH6.0-8.0 |
5 | 1 | reservoir | 100 (mM) | ||
6 | 1 | reservoir | 6.25 (mM) | ||
7 | 1 | reservoir | PEG4000-8000 | 25-38 (%) |