1JZI

Pseudomonas aeruginosa Azurin Re(phen)(CO)3(His83)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-17
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	C 1 2 1
Unit cell lengths	93.950, 50.990, 62.440
Unit cell angles	90.00, 128.10, 90.00

Refinement procedure

Resolution	20.000 * - 1.600 *
R-factor	0.227 *
Rwork	0.226
R-free	0.24800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jze
RMSD bond length	0.010 *
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.680
High resolution limit [Å]	1.600 *	1.620
Rmerge	0.080 *
Number of reflections	28956
<I/σ(I)>	19	4.1
Completeness [%]	98.7	89.8
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	293	PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	drop	imidazole	40 (mM)
3	1	drop		2 (mM)	pH7.2
4	1	reservoir	imidazole	100 (mM)	pH6.0-8.0
5	1	reservoir		100 (mM)
6	1	reservoir		6.25 (mM)
7	1	reservoir	PEG4000-8000	25-38 (%)