1JZH
Pseudomonas aeruginosa Azurin Ru(tpy)(bpy)(His83)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.990, 60.610, 69.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.700 |
| R-factor | 0.269 * |
| Rwork | 0.269 |
| R-free | 0.27700 |
| Structure solution method | 1JZE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.059 * | |
| Number of reflections | 12712 | |
| <I/σ(I)> | 19 | 5.2 |
| Completeness [%] | 98.0 | 91.2 |
| Redundancy | 4.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 293 | PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-30 (mg/ml) | |
| 2 | 1 | drop | imidazole | 40 (mM) | |
| 3 | 1 | drop | 2 (mM) | pH7.2 | |
| 4 | 1 | reservoir | imidazole | 100 (mM) | pH6.0-8.0 |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | 6.25 (mM) | ||
| 7 | 1 | reservoir | PEG4000-8000 | 25-38 (%) |
