1JYS
Crystal Structure of E. coli MTA/AdoHcy Nucleosidase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97913, 0.97898, 0.96379 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 51.080, 133.030, 70.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
Rwork | 0.217 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 23.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.043 | 0.215 |
Total number of observations | 205182 * | |
Number of reflections | 38710 * | |
Completeness [%] | 97.6 | 95.4 * |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 20 * | sodium citrate, CHES, CHAPS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 20 * | sodium citrate, CHES, CHAPS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | sodium citrate | 0.7 (M) | |
3 | 1 | drop | CHES | 100 (mM) | pH8.5 |
4 | 1 | drop | CHAPS detergent | 0.8 (mM) |