1JYO
Structure of the Salmonella Virulence Effector SptP in Complex with its Secretion Chaperone SicP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 133 |
Detector technology | CCD |
Collection date | 2001-01-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0051 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.489, 49.582, 138.142 |
Unit cell angles | 90.00, 121.95, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.224 * |
Rwork | 0.224 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.045 * | 0.243 * |
Total number of observations | 452315 * | |
Number of reflections | 75880 | |
<I/σ(I)> | 21.5 | 3.7 |
Completeness [%] | 96.2 | 74.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 2M sodium chloride, 5-10% polyethylene glycol molecular weight 6000 (PEG6000), supplemented with 2mM DTT and 15% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 60 (mg/ml) | |
2 | 1 | reservoir | 2 (M) | ||
3 | 1 | reservoir | PEG6000 | 5-10 (%) | |
4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
5 | 1 | reservoir | glycerol | 15 (%) |