1JYO
Structure of the Salmonella Virulence Effector SptP in Complex with its Secretion Chaperone SicP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 133 |
| Detector technology | CCD |
| Collection date | 2001-01-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0051 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.489, 49.582, 138.142 |
| Unit cell angles | 90.00, 121.95, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.045 * | 0.243 * |
| Total number of observations | 452315 * | |
| Number of reflections | 75880 | |
| <I/σ(I)> | 21.5 | 3.7 |
| Completeness [%] | 96.2 | 74.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 2M sodium chloride, 5-10% polyethylene glycol molecular weight 6000 (PEG6000), supplemented with 2mM DTT and 15% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 60 (mg/ml) | |
| 2 | 1 | reservoir | 2 (M) | ||
| 3 | 1 | reservoir | PEG6000 | 5-10 (%) | |
| 4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | glycerol | 15 (%) |
