1JYO

Structure of the Salmonella Virulence Effector SptP in Complex with its Secretion Chaperone SicP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	133
Detector technology	CCD
Collection date	2001-01-18
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0051
Spacegroup name	C 1 2 1
Unit cell lengths	167.489, 49.582, 138.142
Unit cell angles	90.00, 121.95, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.224 *
Rwork	0.224
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.045 *	0.243 *
Total number of observations	452315 *
Number of reflections	75880
<I/σ(I)>	21.5	3.7
Completeness [%]	96.2	74.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	2M sodium chloride, 5-10% polyethylene glycol molecular weight 6000 (PEG6000), supplemented with 2mM DTT and 15% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	60 (mg/ml)
2	1	reservoir		2 (M)
3	1	reservoir	PEG6000	5-10 (%)
4	1	reservoir	dithiothreitol	2 (mM)
5	1	reservoir	glycerol	15 (%)