1JYH

Crystal Structure of the Escherichia coli SbmC protein (AKA Gyrase Inhibitory Protein GyrI, AKA YeeB)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-08
Detector	MARRESEARCH
Wavelength(s)	0.97935, 0.97954
Spacegroup name	P 43 21 2
Unit cell lengths	72.440, 72.440, 80.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 * - 1.800
R-factor	0.196
Rwork	0.196
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.057	0.090
Number of reflections	19658
Completeness [%]	95.7	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	295	PEG2000 monomethyl ether, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7 *	295	PEG2000 monomethyl ether, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	0.1 (M)	pH4.6
2	1	reservoir	ammonium sulfate	40 (mM)
3	1	reservoir	PEG2000MME	26 (%(w/v))
4	1	drop	HEPES	20 (mM)	pH7.0
5	1	drop		100 (mM)
6	1	drop	dithiothreitol	3 (mM)