1JYF

Structure of the Dimeric Lac Repressor with an 11-residue C-terminal Deletion.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	91.700, 91.700, 222.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 3.000
R-factor	0.203 *
Rwork	0.203
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Lac repressor dimer
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600
High resolution limit [Å]	3.000	3.000
Rmerge	0.168	0.507
Total number of observations	64464 *
Number of reflections	9811
<I/σ(I)>	4.5	1.5
Completeness [%]	99.4	99.5
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	Sodium Citrate, Sodium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	1.3-1.5 (M)
2	1	reservoir	sodium citrate	0.1 (M)