1JYF
Structure of the Dimeric Lac Repressor with an 11-residue C-terminal Deletion.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 91.700, 91.700, 222.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 3.000 |
R-factor | 0.203 * |
Rwork | 0.203 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Lac repressor dimer |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.168 | 0.507 |
Total number of observations | 64464 * | |
Number of reflections | 9811 | |
<I/σ(I)> | 4.5 | 1.5 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | Sodium Citrate, Sodium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium acetate | 1.3-1.5 (M) | |
2 | 1 | reservoir | sodium citrate | 0.1 (M) |