1JYA
Crystal Structure of SycE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Detector technology | CCD |
| Collection date | 2001-03-30 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.000, 75.190, 137.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.740 |
| R-factor | 0.236 * |
| Rwork | 0.236 |
| R-free | 0.26600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.010 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.056 | 0.365 |
| Number of reflections | 46896 | |
| <I/σ(I)> | 19 | 4.4 |
| Completeness [%] | 93.6 | 99.6 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 18 * | pH 8.5, temperature 100K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
| 2 | 1 | reservoir | 300 (mM) | ||
| 3 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | drop | protein | 40 (mg/ml) |
