1JY5
RNase-related protein from Calystegia sepium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9116 |
Spacegroup name | P 62 |
Unit cell lengths | 121.687, 121.687, 66.059 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.630 - 2.050 |
R-factor | 0.2074 * |
Rwork | 0.207 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dix |
RMSD bond length | 0.005 |
RMSD bond angle | 1.230 * |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.063 | 0.338 |
Total number of observations | 163993 * | |
Number of reflections | 35416 * | 1739 * |
<I/σ(I)> | 20.71 | 5.12 |
Completeness [%] | 85.5 | 66.3 * |
Redundancy | 4.63 | 4.03 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | NaCl, 0.1M acetate buffer, MPD, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 0.2 (M) | ||
2 | 1 | drop | acetate | 0.1 (M) | pH4.6 |
3 | 1 | drop | MPD | 30 (%) | |
4 | 1 | drop | protein | 10 (mg/ml) |