1JY2
Crystal Structure of the Central Region of Bovine Fibrinogen (E5 fragment) at 1.4 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.376, 66.222, 50.658 |
| Unit cell angles | 90.00, 106.55, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.400 |
| R-factor | 0.216 * |
| Rwork | 0.216 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1.6 Angstrom resolution structure of bovine E5 in the orthorhombic space group |
| RMSD bond length | 0.011 |
| RMSD bond angle | 19.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.058 * | 0.334 * |
| Total number of observations | 359638 * | |
| Number of reflections | 61149 | |
| Completeness [%] | 99.2 | 93.1 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 4 * | PEG 3350, Calcium chloride, Tris, Dioxane, Sodium azide, pH 8.0, VAPOR DIFFUSION, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 15.0 (%) | |
| 3 | 1 | reservoir | 150 (mM) | ||
| 4 | 1 | reservoir | dioxane | 3.5 (%) | |
| 5 | 1 | reservoir | 3 (mM) | ||
| 6 | 1 | reservoir | Tris-HCl | 20 (mM) | pH8.0 |
