1JXV

Crystal Structure of Human Nucleoside Diphosphate Kinase A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	1997
Detector	WEISSENBERG
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	74.210, 78.110, 82.290
Unit cell angles	90.00, 101.33, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.207 *
Rwork	0.207
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nue
RMSD bond length	0.009
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.150
Rmerge	0.060
Number of reflections	48679
<I/σ(I)>	19.1
Completeness [%]	90.9
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.35	288 *	MPD, MES-KOH, pH 6.35, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	MES/KOH	100 (mM)
3	1	reservoir	MPD	30-34 (%(v/v))	pH6.35