1JXK
Role of ethe mobile loop in the mehanism of human salivary amylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.900, 75.737, 134.554 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.420 - 1.900 |
R-factor | 0.17615 |
Rwork | 0.173 |
R-free | 0.19997 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1smd |
RMSD bond length | 0.008 |
RMSD bond angle | 1.600 * |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.420 | 1.949 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.057 | 0.057 |
Total number of observations | 174537 * | |
Number of reflections | 38401 | |
<I/σ(I)> | 21.9 | 8.6 |
Completeness [%] | 98.3 | 99 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | MPD, calcium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | reservoir | MPD | 40 (%(v/v)) |