1JXA
GLUCOSAMINE 6-PHOSPHATE SYNTHASE WITH GLUCOSE 6-PHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.400, 112.400, 185.100 |
| Unit cell angles | 90.00, 96.40, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.100 |
| R-factor | 0.2 * |
| Rwork | 0.200 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1moq 1GDO |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.056 | |
| Total number of observations | 111045 * | |
| Number of reflections | 45685 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 98.5 | 92 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | Obmolova, G., (1994) J.Mol.Biol., 242, 703. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 0.1 (M) | |
| 3 | 1 | drop | 1 (M) | ||
| 4 | 1 | drop | PEG4000 | 3 (%(w/v)) | |
| 5 | 1 | drop | Fru6P | 10 (mM) |
