1JWY
CRYSTAL STRUCTURE OF THE DYNAMIN A GTPASE DOMAIN COMPLEXED WITH GDP, DETERMINED AS MYOSIN FUSION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.550, 61.950, 181.070 |
| Unit cell angles | 90.00, 94.52, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.300 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JX2 (SAME PROTEIN WITHOUT NUCLEOTIDE) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.054 * | 0.186 * |
| Number of reflections | 50492 | |
| <I/σ(I)> | 15.7 | 4.1 |
| Completeness [%] | 94.0 | 53.6 |
| Redundancy | 3.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 8000, Tris, potassium chloride, magnesium chloride, glucose, methyl-propane-diol, dithiothreitol, EGTA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 11 (%) | |
| 10 | 1 | drop | 1 (mM) | ||
| 11 | 1 | drop | dithiothreitol | 1 (mM) | |
| 12 | 1 | drop | sucrose | 3 (%) | |
| 13 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 50 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | glucose | 10 (%) | |
| 6 | 1 | reservoir | MPD | 2 (%) | |
| 7 | 1 | reservoir | EGTA | 1 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 9 | 1 | drop | Tris | 50 (mM) |
