1JWR

Crystal structure of human lysozyme at 100 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.170, 60.980, 33.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.400
R-factor	0.178 *
Rwork	0.178
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rex
RMSD bond length	0.011
RMSD bond angle	24.106 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.048	0.115
Total number of observations	184173 *
Number of reflections	22814
<I/σ(I)>	44.6	11.3
Completeness [%]	98.4	94.9
Redundancy	8.07	7.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	10 *	Takano, K., (1995) J. Mol. Biol., 254, 62. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		2.5 (M)
3	1	reservoir	acetate	20 (mM)