1JWL
Structure of the Dimeric lac Repressor/Operator O1/ONPF Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 255.771, 255.771, 206.795 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.990 - 4.000 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.000 | |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.111 * | 0.277 * |
| Total number of observations | 68073 * | |
| Number of reflections | 20847 | |
| <I/σ(I)> | 7.9 | 2.7 |
| Completeness [%] | 96.1 | 93.9 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 22 * | ammonium sulfate, PEG400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 2 | 1 | 1 | PEG400 | ||
| 3 | 1 | 1 | HEPES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | protein | 10 (mg/ml) | ||
| 2 | 1 | 2 | ammonium sulfate | 1.8 (M) | |
| 3 | 1 | 2 | PEG400 | 8.2 (%(w/v)) | |
| 4 | 1 | 2 | HEPES | 0.1 (M) |
