1JWA

Structure of the ATP-bound MoeB-MoaD Protein Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-24
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 41 21 2
Unit cell lengths	78.723, 78.723, 103.184
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.203 *
Rwork	0.203
R-free	0.26756
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.019
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.020
High resolution limit [Å]	2.900	2.900
Rmerge	0.143	0.362
Number of reflections	7355
<I/σ(I)>	9.4	2.4
Completeness [%]	98.9	100
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	Lithium Sulfate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MoeB	23 (mg/ml)
2	1	drop	MoaD	10 (mg/ml)
3	1	reservoir		1.7 (M)
4	1	reservoir	HEPES	100 (mM)