1JW9
Structure of the Native MoeB-MoaD Protein Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.832, 77.832, 102.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.175 * |
| Rwork | 0.175 |
| R-free | 0.20856 |
| Structure solution method | MIR |
| RMSD bond length | 0.021 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.710 |
| Rmerge | 0.087 | 0.408 |
| Number of reflections | 37865 | |
| <I/σ(I)> | 17.5 | 1.9 |
| Completeness [%] | 99.7 * | 99.3 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | Lithium Sulfate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MoeB | 23 (mg/ml) | |
| 2 | 1 | drop | MoaD | 10 (mg/ml) | |
| 3 | 1 | reservoir | 1.7 (M) | ||
| 4 | 1 | reservoir | HEPES | 100 (mM) |
