1JVX
Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-02 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.610, 67.580, 57.270 |
Unit cell angles | 90.00, 113.31, 90.00 |
Refinement procedure
Resolution | 27.560 - 2.500 |
R-factor | 0.232 * |
Rwork | 0.232 |
R-free | 0.30400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mdq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9999.000 * | 3.000 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.066 | |
Total number of observations | 24731 * | |
Number of reflections | 12533 | |
<I/σ(I)> | 8.1 | 3.6 |
Completeness [%] | 98.7 | 99 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 4 * | PEG 6000, sodium MES, maltose, sodium azide, beta-mercaptoethanol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5-10 (mg/ml) | |
2 | 1 | drop | PEG6000 | 9-15 (%(w/v)) | |
3 | 1 | drop | MES-Na | 10 (mM) | |
4 | 1 | drop | maltose | 1 (mM) | |
5 | 1 | drop | sodium azide | 0.02 (%) | |
6 | 1 | reservoir | PEG6000 | 19-22 (%) |