1JVX
Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.610, 67.580, 57.270 |
| Unit cell angles | 90.00, 113.31, 90.00 |
Refinement procedure
| Resolution | 27.560 - 2.500 |
| R-factor | 0.232 * |
| Rwork | 0.232 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mdq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | 3.000 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.066 | |
| Total number of observations | 24731 * | |
| Number of reflections | 12533 | |
| <I/σ(I)> | 8.1 | 3.6 |
| Completeness [%] | 98.7 | 99 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 4 * | PEG 6000, sodium MES, maltose, sodium azide, beta-mercaptoethanol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5-10 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 9-15 (%(w/v)) | |
| 3 | 1 | drop | MES-Na | 10 (mM) | |
| 4 | 1 | drop | maltose | 1 (mM) | |
| 5 | 1 | drop | sodium azide | 0.02 (%) | |
| 6 | 1 | reservoir | PEG6000 | 19-22 (%) |
