1JVW

TRYPANOSOMA CRUZI MACROPHAGE INFECTIVITY POTENTIATOR (TCMIP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12-20
Detector	MARRESEARCH
Wavelength(s)	0.885
Spacegroup name	P 1 21 1
Unit cell lengths	46.034, 34.587, 50.412
Unit cell angles	90.00, 91.57, 90.00

Refinement procedure

Resolution	19.000 - 1.700
R-factor	0.179
Rwork	0.176
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.572
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.200	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.081 *	0.097 *
Total number of observations	60547 *
Number of reflections	17009
Completeness [%]	95.9	89.8
Redundancy	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5	4 *	PMSF, Tris/HCl, pH 7.5, SMALL TUBES, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	5 (mM)	pH7.5
2	1	1	PMSF	1 (mM)