1JVV
CRYSTAL STRUCTURE OF RIBONUCLEASE A (RETRO-SOAKED FORM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-22 |
| Detector | MAC Science DIP-2030B |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.270, 33.200, 73.580 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.18 |
| Rwork | 0.170 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jvu |
| RMSD bond length | 0.017 |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.086 | |
| Total number of observations | 29907 * | |
| Number of reflections | 11257 | |
| Completeness [%] | 89.0 | 87 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PEG 4000, sodium citrate, pH 5.00, VAPOR DIFFUSION, HANGING DROP at 298K. THE CRYSTAL WAS PREVIOUSLY SOAKED WITH 2'-CMP AND THEN RETRO-SOAKED BY WASHING OUT THE NUCLEOTIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 10 (mM) | pH5.0 |
| 3 | 1 | drop | PEG4000 | 10 (%(w/v)) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 5 | 1 | reservoir | sodium citrate | 20 (mM) | pH5.0 |
