1JVD

CRYSTAL STRUCTURE OF HUMAN AGX2 COMPLEXED WITH UDPGLCNAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-20
Detector	MARRESEARCH
Wavelength(s)	0.9310
Spacegroup name	P 1 21 1
Unit cell lengths	86.543, 71.281, 91.390
Unit cell angles	90.00, 92.82, 90.00

Refinement procedure

Resolution	20.000 * - 2.400
Rwork	0.193
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.400
Rmerge	0.062 *	0.273 *
Number of reflections	43625
<I/σ(I)>	6.2
Completeness [%]	99.8	99.8 *
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	20 *	PEG600, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.000001 (mM)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop		20 (mM)
4	1	drop		5 (mM)
5	1	reservoir	PEG600	34 (%)
6	1	reservoir	imidazole/malate	0.2 (M)	pH5.7