1JV5

Anti-blood group A Fv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	300
Detector technology	DIFFRACTOMETER
Collection date	1994-10-15
Detector	ENRAF-NONIUS FAST
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	51.710, 57.960, 83.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.183
Rwork	0.183
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.412
Data reduction software	ROTAVATA
Data scaling software	CCP4 ((AGROVATA)
Phasing software	MERLOT
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.310
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.073
Total number of observations	24752 *
Number of reflections	12348 *
<I/σ(I)>		9.3
Completeness [%]	93.0	71.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	300	Patenaude, S.I., (1998) Acta Crystallogr., Sect.D, 54, 1456. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fv	7 (mg/ml)
2	1	reservoir	PEG4000	25-30 (%)
3	1	reservoir	HEPES	0.1 (M)	pH8.0
4	1	reservoir		10 (mM)