1JV3

CRYSTAL STRUCTURE OF HUMAN AGX1 COMPLEXED WITH UDPGALNAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9330
Spacegroup name	P 1 21 1
Unit cell lengths	85.735, 70.769, 95.384
Unit cell angles	90.00, 95.26, 90.00

Refinement procedure

Resolution	25.000 * - 2.200
Rwork	0.190
R-free	0.23300 *
Structure solution method	rigid body
Starting model (for MR)	pdb code: 1JV1
RMSD bond length	0.011
RMSD bond angle	24.000 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.066 *	0.373 *
Number of reflections	64858
<I/σ(I)>	7.4823	1.84
Completeness [%]	97.6	98.2 *
Redundancy	2.62	2.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	20 *	peg600, Imidazole/malate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.000001 (mM)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop		20 (mM)
4	1	drop		5 (mM)
5	1	reservoir	PEG600	34 (%)
6	1	reservoir	imidazole/malate	0.2 (M)	pH5.7