1JV1

CRYSTAL STRUCTURE OF HUMAN AGX1 COMPLEXED WITH UDPGLCNAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	85.829, 70.904, 96.180
Unit cell angles	90.00, 95.25, 90.00

Refinement procedure

Resolution	25.000 * - 1.900
Rwork	0.183
R-free	0.22000
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	24.300 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	SnB
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.840	1.950
High resolution limit [Å]	1.900 *	1.900
Rmerge	0.056 *	0.305 *
Number of reflections	90423
<I/σ(I)>	10.6	2.2
Completeness [%]	99.6	97.4
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	20 *	PEG600, Imidazole/Malate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.000001 (mM)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop		20 (mM)
4	1	drop		5 (mM)
5	1	reservoir	PEG600	34 (%)
6	1	reservoir	imidazole/malate	0.2 (M)	pH5.7