1JV1
CRYSTAL STRUCTURE OF HUMAN AGX1 COMPLEXED WITH UDPGLCNAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.829, 70.904, 96.180 |
| Unit cell angles | 90.00, 95.25, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.900 |
| Rwork | 0.183 |
| R-free | 0.22000 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 24.300 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.840 | 1.950 |
| High resolution limit [Å] | 1.900 * | 1.900 |
| Rmerge | 0.056 * | 0.305 * |
| Number of reflections | 90423 | |
| <I/σ(I)> | 10.6 | 2.2 |
| Completeness [%] | 99.6 | 97.4 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 20 * | PEG600, Imidazole/Malate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.000001 (mM) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | reservoir | PEG600 | 34 (%) | |
| 6 | 1 | reservoir | imidazole/malate | 0.2 (M) | pH5.7 |
