1JUX
1.6A Resolution Crystal Structures of the DNA Octamers d(IUATATAC) and d(ITITACAC):Binding of Two Distamycin Drugs Side-by-Side
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06-20 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 33.260, 25.050, 28.300 |
Unit cell angles | 90.00, 124.23, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.600 |
Rwork | 0.149 |
R-free | 0.20700 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.300 * |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | |
High resolution limit [Å] | 1.600 | 1.600 * |
Rmerge | 0.046 * | 0.162 * |
Number of reflections | 2737 * | 381 * |
Completeness [%] | 92.0 * | 88.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 1 (mM) | single-stranded concentration |
2 | 1 | drop | sodium cacodylate | 30 (mM) | pH7.0 |
3 | 1 | drop | cobalt hexammine chloride | 0.1 (mM) | |
4 | 1 | drop | distamycin hydrochloride | 1 (mM) | |
5 | 1 | reservoir | MPD | 60 (%) |