1JUO

Crystal Structure of Calcium-free Human Sorcin: A Member of the Penta-EF-Hand Protein Family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-11-30
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	118.250, 118.250, 85.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.560 - 2.200
R-factor	0.212 *
Rwork	0.218
R-free	0.25600 *
Structure solution method	MIR
RMSD bond length	0.007 *
RMSD bond angle	1.199 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNX (2000)
Refinement software	CNX (2000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.064	0.491
Total number of observations	167295 *
Number of reflections	28472
<I/σ(I)>		2.1
Completeness [%]	90.7	75.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	sodium chloride, HEPES, EGTA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sorcin	0.004 (mM)
2	1	drop	fluo-4 pentapossium salt	0.010 (mM)
3	1	drop	urea	6.4 (mM)	or 7.8M
4	1	reservoir		0.5 (M)
5	1	reservoir	HEPES	0.1	pH7.0
6	1	reservoir	EGTA	2.5 (mM)