1JUK
INDOLE-3-GLYCEROLPHOSPHATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS IN A TRIGONAL CRYSTAL FORM
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-12-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.400, 62.400, 122.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1igs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.780 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.400 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.093 | 0.295 |
| Number of reflections | 9631 | |
| <I/σ(I)> | 7.1 | 2.5 |
| Completeness [%] | 95.5 | 50.6 |
| Redundancy | 4.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 4 * | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG5000 | 15 (%(w/v)) | |
| 3 | 1 | drop | ammonium sulfate | 100 (mM) | |
| 4 | 1 | drop | MES | 0.05 (M) | |
| 5 | 1 | reservoir | PEG5000 | 30 (%(w/v)) | |
| 6 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 7 | 1 | reservoir | MES | 0.1 (M) |
