1JUF
Structure of Minor Histocompatibility Antigen peptide, H13b, complexed to H2-Db
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.997, 109.707, 57.654 |
| Unit cell angles | 90.00, 120.52, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.000 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.24200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1inq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 32587 | |
| <I/σ(I)> | 47.6 | 15.8 |
| Completeness [%] | 97.3 | 75.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4000, Sodium Acetates, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
