1JUB
The K136E mutant of lactococcus lactis dihydroorotate dehydrogenase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-11-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.880, 108.250, 65.730 |
| Unit cell angles | 90.00, 103.74, 90.00 |
Refinement procedure
| Resolution | 13.000 - 1.400 |
| Rwork | 0.191 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LACTOCOCCUS LACTIS DHODA PDB ID 1DOR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.410 |
| Rmerge | 0.040 | 0.280 |
| Number of reflections | 133686 | |
| <I/σ(I)> | 21.5 | 3.4 |
| Completeness [%] | 96.9 | 98.5 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 6K, Na-acetate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
