1JU2
Crystal structure of the hydroxynitrile lyase from almond
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1999-06-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.176, 67.494, 79.796 |
| Unit cell angles | 79.57, 77.78, 67.19 |
Refinement procedure
| Resolution | 25.000 * - 1.470 |
| Rwork | 0.160 |
| R-free | 0.18800 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.045 | |
| Total number of observations | 316821 * | |
| Number of reflections | 128976 | |
| <I/σ(I)> | 23.6 | 2.1 |
| Completeness [%] | 68.3 | 30.5 |
| Redundancy | 2.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 295 | PEG 4000, isopropanol, NaHepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | isopropanol | 16 (%) | |
| 3 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | drop | protein | 12 (mg/ml) | |
| 5 | 1 | drop | citrate | 10 (mM) | pH5.5 |
