1JTZ
CRYSTAL STRUCTURE OF TRANCE/RANKL CYTOKINE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.300, 82.000, 99.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHIMERA OF PDB ENTRIES 1TNR (CHAIN A) AND 1D4V (CHAIN B) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.449 * | |
| Total number of observations | 191355 * | |
| Number of reflections | 16705 | |
| <I/σ(I)> | 32.3 | 4.7 |
| Completeness [%] | 98.2 | 100 |
| Redundancy | 11.5 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | 11-14% PEG 4000, 16 mM calcium chloride, 80 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | reservoir | HEPES | 80 (mM) | |
| 5 | 1 | reservoir | 16 (mM) | ||
| 6 | 1 | reservoir | PEG4000 | 11-14 (%) |
