1JTN
Alternative Structures of a Sequence Extended T4 Lysozyme Show that the Highly Conserved Beta-Sheet Region has weak intrinsic Folding Propensity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.773 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.125, 32.314, 85.925 |
| Unit cell angles | 90.00, 102.64, 90.00 |
Refinement procedure
| Resolution | 19.600 - 2.300 |
| R-factor | 0.221 * |
| Rwork | 0.220 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2LZM |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.043 * | |
| Number of reflections | 14476 | |
| <I/σ(I)> | 13.6 | 7 |
| Completeness [%] | 97.6 * | 98.9 |
| Redundancy | 3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-glycine | 50 (mM) | pH7.5 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 18 (%) | |
| 5 | 1 | reservoir | lithium sulfate | 200 (mM) | |
| 6 | 1 | reservoir | Tris-glycine | 50 (mM) | pH6.8 |
