Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1JTM

Alternative Structures of a Sequence Extended T4 Lysozyme Show that the Highly Conserved Beta-Sheet has Weak Intrinsic Folding Propensity

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]170
Detector technologyIMAGE PLATE
Collection date1999-07-21
DetectorRIGAKU RAXIS IV
Wavelength(s)1.5418
Spacegroup nameP 32 2 1
Unit cell lengths60.970, 60.970, 97.345
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution17.000 - 1.900
R-factor0.214

*

Rwork0.214
R-free0.26500
Starting model (for MR)PDB ID 2LZM
RMSD bond length0.010
RMSD bond angle1.400

*

Data reduction softwareMOSFLM
Data scaling softwareCCP4 ((SCALA))
Refinement softwareTNT
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]17.0002.010
High resolution limit [Å]1.9001.900
Rmerge0.068

*

Number of reflections19736
<I/σ(I)>3.92.6
Completeness [%]99.899.8
Redundancy44
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5

*

298used microseeding

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropTris-glycine50 (mM)pH7.5
21drop100 (mM)
31dropprotein20 (mg/ml)
41reservoirPEG800020 (%(w/v))
51reservoirisopropanol10 (%(v/v))
61reservoirphosphate50 (mM)pH7.0

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon