1JTL
The crystal structure of d(GGCCAATTGG) Complexed with Distamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84230 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.289, 36.439, 53.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.850 |
| R-factor | 0.1974 |
| R-free | 0.27800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DD0002 used as starting model for refinement |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 2.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.049 | 0.195 * |
| Total number of observations | 43223 * | |
| Number of reflections | 4223 | |
| <I/σ(I)> | 21.08 | 5.1 |
| Completeness [%] | 93.0 | 96.9 |
| Redundancy | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 * | 20 * | MPD, magnesium chloride, cacodylate, spermine, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 1 | spermine |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | 25 (mM) | ||
| 3 | 1 | drop | sodium acetate | 50 (mM) | pH5. |
| 4 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
