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1JTL

The crystal structure of d(GGCCAATTGG) Complexed with Distamycin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsEMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron siteEMBL/DESY, HAMBURG
BeamlineBW7B
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2000-04-18
DetectorMARRESEARCH
Wavelength(s)0.84230
Spacegroup nameP 21 21 21
Unit cell lengths25.289, 36.439, 53.047
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution100.000 - 1.850
R-factor0.1974
R-free0.27800

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Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)DD0002 used as starting model for refinement
RMSD bond length0.019

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RMSD bond angle2.700

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Refinement softwareSHELXL
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]100.0001.920
High resolution limit [Å]1.8501.850
Rmerge0.0490.195

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Total number of observations43223

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Number of reflections4223
<I/σ(I)>21.085.1
Completeness [%]93.096.9
Redundancy4.33.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5

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20

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MPD, magnesium chloride, cacodylate, spermine, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111MPD
211MgCl2
311cacodylate
411spermine
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein9 (mg/ml)
21drop25 (mM)
31dropsodium acetate50 (mM)pH5.
41reservoirammonium sulfate0.4 (M)
51reservoirsodium acetate0.1 (M)pH4.5

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PDB entries from 2024-11-06

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