1JTI
Loop-inserted Structure of P1-P1' Cleaved Ovalbumin Mutant R339T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-11-15 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.714, 65.465, 68.422 |
| Unit cell angles | 89.11, 63.27, 61.72 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.25200 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.384 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.049 * | |
| Total number of observations | 98045 * | |
| Number of reflections | 36122 | |
| Completeness [%] | 95.7 | 93.3 |
| Redundancy | 2.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 30 * | ammonium sulfate, Bis-Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 10 (mM) | pH6.0 |
| 3 | 1 | reservoir | ammonium sulfate | 47-48 (%) | |
| 4 | 1 | reservoir | Bis-Tris | 0.05 (M) | pH7.0 |
