1JTE
Crystal Structure Analysis of VP39 F180W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 1998-11-02 |
| Detector | SIEMENS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.640, 67.940, 80.020 |
| Unit cell angles | 90.00, 117.92, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.242 |
| Rwork | 0.232 |
| R-free | 0.28500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.430 * |
| Data reduction software | SAINT ((SIEMENS)) |
| Data scaling software | SAINT ((SIEMENS)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 * | |
| Number of reflections | 23570 | |
| Completeness [%] | 87.5 | 50 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.5 | 298 | Hodel, A.E., (1996) Cell(Cambridge,Mass.), 85, 247. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 10 (%) | |
| 2 | 1 | 1 | 0.125 (M) | ||
| 3 | 1 | 1 | citrate | 0.1 (M) |
