1JT0
Crystal structure of a cooperative QacR-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 |
| Unit cell lengths | 174.700, 174.700, 151.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.700 * - 2.900 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25800 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.730 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.054 | 0.401 * |
| Total number of observations | 302270 * | |
| Number of reflections | 59396 | |
| <I/σ(I)> | 11.4 | 1.7 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 298 | Lithium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Li2(SO4) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | lithium sulfate | 100 (%sat) | |
| 2 | 1 | reservoir | magnesium sulfate | 10 (mM) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
