1JSX
Crystal Structure of the Escherichia coli Glucose-Inhibited Division Protein B (GidB)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2000-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97903, 0.97927 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 53.914, 53.914, 150.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 * - 2.400 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.27500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 9170 | |
| Completeness [%] | 91.8 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 4 * | 15% PEG4000, 10% isopropanol, 0.1M sodium citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 4 * | 15% PEG4000, 10% isopropanol, 0.1M sodium citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 20 (mM) | pH7.0 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 3 (mM) | |
| 4 | 1 | drop | protein | 24 (mg/ml) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH6.5 |
| 6 | 1 | reservoir | PEG4000 | 15 (%) | |
| 7 | 1 | reservoir | isopropanol | 10 (%) |
